A potential of Jasminum sambac (L.) Aiton leaf nano-extract as spray treatment of gingivitis-induced Sprague Dawley rats

Gingivitis is the second most common disease that occurs in oral cavity. The use of chlorhexidine as a gingivitis therapy agent has some side effects such allergic reactions and bacterial resistance. Jasminum Sambac (L.) Aiton leaf nano-extract contains flavonoids, saponins, tannins compounds reported to have an influence on wound healing process. aim this research was determine effect observation time concentrations sambac number neutrophils, macrophages, angiogenesis treatment gingivitis-induced Sprague Dawley rats. Sixty male rats aged 2.5-3 months with body weight 200-250 g were anesthetized ketamine HCl (10 mg/kg BW) xylazine (2 then induced using silk ligature 3.0 interdental mandibular incisor. divided into five groups got daily spray distilled water (negative control), at concentration 40%, 45%, 50%, 0.12% (positive control). euthanized 1st, 3rd, 5th, 7th days post-treatment. tissues processed histologically HE staining. counted microscope (400x) OptiLab Viewer® (13x) fields view. result Two Way ANOVA test showed there significant time, interaction both (p < 0.05). It can be concluded affected macrophage, rat